Product
CellCount™ Cell Counting Kit-8
CellCount™ Cell Counting Kit-8
CellCount™ Cell Counting Kit-8 utilizes a water-soluble tetrazolium salt to measure cell viability in a non-radioactive, colorimetric assay. The reagent offers excellent stability and low cytotoxicity, making CCK-8 an ideal choice for cell proliferation and cytotoxicity studies.
The kit is available in two formats: a ready-to-use solution (CC02) and a lyophilized powder (CC03) for enhanced storage flexibility.
產品資訊
Highlights
- Applications: Ideal for cell proliferation and cytotoxicity assays
- Detection: Colorimetric measurement at 450 nm absorbance
- Lyophilized format: Enhanced stability for long-term storage (CC03)
Order Information
Storage
Shipped at room temperature; store at 2–8 °C
Principle of the MTT Assay
Figure 1. CCK-8 (WST-8) assay diagram
The CCK-8 assay utilizes WTS-8, a high-purity, water-soluble tetrazolium salt that reacts with intracellular dehydrogenases in viable cells. This redox reaction reduces WTS-8 to an orange-colored formazan product, which is directly soluble in the culture medium. The amount of formazan generated is proportional to the number of living cells and can be quantitatively measured by absorbance at 450 nm.
Figure 2. Operation of CCK-8 assay.
If using CCK-8 powder (#CC03-S / CC03-01 / CC03-10), please dissolve the powder in double-distilled water (ddH₂O) before performing the CCK-8 assay.
Reference
| Item | Description | LINK |
| 1 |
Hsu, Wan-Yi, et al. "Prediction of miRNA‑mRNA network regulating the migration ability of cytarabine‑resistant HL60 cells." Biomedical Reports 20.2 (2023): 20.
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LINK |
| 2 |
Wu, Ming-Ju, et al. "Targeting mitophagy using isoliensinine as a therapeutic strategy for renal cell carcinoma treatment." Free Radical Biology and Medicine 233 (2025): 132-147.
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PMID: 43139412 |