Product
ImmunoFast™ Adjuvant
ImmunoFast™ Adjuvant is an innovative aqueous-based emulsified adjuvant designed to rapidly stimulate a strong immune response and promote high IgG antibody production within a short time frame.
Its water-based formulation makes antigen mixing and injection more convenient, while the non-toxic, animal-friendly composition improves the survival rate of immunized animals throughout the experimental period.
Highlights
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Strong – Elicits a robust immune response with reduced antigen dose
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Effective – Promotes high-efficiency antibody production and complete class switching from IgM to IgG
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Simple – Easy to mix, thanks to its aqueous-based formulation
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Safe – Non-toxic and well-tolerated by immunized animals
Order Information
Cat. No. | Product Name | Description |
IF01-4N | ImmunoFast™ Adjuvant |
For 4 injections for mouse |
IF01-20N | ImmunoFast™ Adjuvant |
For 20 injections for mouse |
FAQs:
Q1 : How much antigen should be used while immunizing animals?
Q1 : Generally, 10-50μg of antigen in each boost should be sufficient for successful immunization. However, due to the divergence among antigens, the optimal amount of required antigen might vary and should be determined by users.
Q2 : What should I note while mixing the antigen with ImmunoFast Adjuvant?
A2 : Mix the antigen with ImmunoFast Adjuvant thoroughly just for 5 minutes, then inject into the animals. Compared to conventional adjuvant (CFA or IFA), the emulsifying solution from ImmunoFast is water like and thus provides users an easier immunization procedure.
Q3 : Whether the immunoresponse can be induced when the antigen size is only 10~15 kD?
A3 : According to our experiments, the immunoresponse can be successfully induced when the antigen is only 10-15 kDa. However, due to differences in antigen characteristics, results may vary and should be verified for each specific case.
Reference:
1. Liu, C. F. & Pan, T. M. Recombinant expression of bioactive peptide lunasin in Escherichia coli. Appl Microbiol Biotechnol 88, 177-186 (2010).
Antigen: his-tagged lunasin (11.35 kDa); animal: mice
2. Shin, Y. C. et al. Biochemical characterization of the small ubiquitin-like modifiers of Chlamydomonas reinhardtii. Planta 232, 649-662 (2010).
Antigen: his-tagged CrSUMO (~17 kDa); animal: rat